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Culture - duodenal tissue

Definition

A duodenal tissue culture is a laboratory exam to check a piece of tissue from the first part of the small intestine (duodenum). The test is to look for organisms that cause infection.

Alternative Names

Duodenal tissue culture

How the Test is Performed

A piece of tissue from the first part of the small intestine is taken during an upper endoscopy (esophagogastroduodenoscopy).

The sample is then sent to a lab. There it is placed in a special dish (culture media) that allows bacteria or viruses to grow. The sample is looked at under a microscope regularly to see if any organisms are growing.

Organisms that grow on the culture are identified.

How to Prepare for the Test

This is a test done in a lab. The sample is collected during an upper endoscopy and biopsy procedure (esophagogastroduodenoscopy). Ask your health care provider how to prepare for this procedure.

Why the Test is Performed

A culture of duodenal tissue is done to check for bacteria or viruses that may lead to certain illnesses and conditions.

Normal Results

No harmful bacteria or viruses are found.

What Abnormal Results Mean

An abnormal finding means that harmful bacteria or a virus has been found in the tissue sample. Bacteria may include:

  • Campylobacter
  • Helicobacter pylori
  • Salmonella

Considerations

Other tests are very often done to look for infection-causing organisms in duodenal tissue. These tests include the urease test (for example, the CLO test) and histology (looking at the tissue under a microscope).

Routine culture for H pylori is not currently recommended.

References

Beavis KG, Charnot-Katsikas A. Specimen collection and handling for diagnosis of infectious diseases. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd ed. Philadelphia, PA: Elsevier; 2017:chap 64.

Dupont HL. Approach to the patient with suspected enteric infection. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 283.

Fritsche TR, Pritt BS. Medical parasitology. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd ed. Philadelphia, PA: Elsevier; 2017:chap 63.

Haines CF, Sears CL. Infectious enteritis and proctocolitis. In: Feldman M, Friedman LS, Brandt LJ, eds. Sleisenger and Fordtran's Gastrointestinal and Liver Disease. 10th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 110.

Semrad CE. Approach to the patient with diarrhea and malabsorption. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine. 25th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 140.

Siddiqi HA, Salwen MJ, Shaikh MF, Bowne WB. Laboratory diagnosis of gastrointestinal and pancreatic disorders In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd ed. Philadelphia, PA: Elsevier; 2017:chap 22.

Review Date:5/11/2016
Reviewed By:Subodh K. Lal, MD, gastroenterologist with Gastrointestinal Specialists of Georgia, Austell, GA. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.

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Outcome Data

No data available for this condition/procedure.

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