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Fetal-maternal erythrocyte distribution

Definition

The fetal-maternal erythrocyte distribution test is used to measure the number of the unborn baby's red blood cells in a pregnant woman's blood.

See also: Rh incompatibility

Alternative Names

Kleihauer-Betke stain

How the Test is Performed

A blood sample is needed.

How to Prepare for the Test

No special preparation is necessary for this test.

How the Test will Feel

When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon goes away.

Why the Test is Performed

Rh incompatibility is a condition that occurs when the mother's blood type is Rh-negative (Rh-) and her unborn baby's blood type is Rh-positive (Rh+). If the mother is Rh+, or if both parents are Rh-, there is no reason to worry about Rh incompatibility.

If the baby's blood is Rh+ and gets into the mother's Rh- bloodstream, her body will produce antibodies. These antibodies could pass back through the placenta and harm the developing baby's red blood cells. This can cause mild to serious anemia in the unborn baby.

This test determines the amount of blood that has been exchanged between the mother and fetus. All Rh- pregnant women should get this test if they have bleeding or a risk of bleeding during the pregnancy.

In a woman whose blood is Rh incompatible with her infant, this test helps find out how much Rh immune globulin (RhoGAM) she must receive to prevent her body from producing abnormal proteins that attack the unborn baby in future pregnancies.

Normal Results

In a normal value, no or few of the baby's cells are in the mother's blood. The standard dose of RhoGAM is enough in this case.

Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.

What Abnormal Results Mean

In an abnormal test result, blood from the unborn baby is leaking into the mother's blood circulation. The more of the baby's cells there are, the more Rh immune globulin the mother must receive.

Risks

Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

Other risks associated with having blood drawn are slight, but may include:

  • Excessive bleeding
  • Fainting or feeling light-headed
  • Hematoma (blood accumulating under the skin)
  • Infection (a slight risk any time the skin is broken)

References

Borawski D, Bluth MH. Reproductive function and pregnancy. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 25.

Matthews DC, Glader B. Erythrocyte disorders in infancy. In: Gleason CA, Devaskar SU, eds. Avery's Diseases of the Newborn. 9th ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 77.

Review Date:8/30/2014
Reviewed By:Neil K. Kaneshiro, MD, MHA, Clinical Assistant Professor of Pediatrics, University of Washington School of Medicine, Seattle, WA. Also reviewed by David Zieve, MD, MHA, Isla Ogilvie, PhD, and the A.D.A.M. Editorial team.

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